A combination of polyunsaturated fatty acid, nonribosomal peptide and polyketide biosynthetic machinery is used to assemble the zeamine antibiotics† †Electronic supplementary information (ESI) available: Complete description of experimental details, and additional tables, figures, chromatograms, spectra, results from biological assays and LC-MS(/MS)- and NMR data for the prezeamines. See DOI: 10.1039/c4sc01927j Click here for additional data file.

A combination of polyunsaturated fatty acid, nonribosomal peptide and polyketide biosynthetic machinery is used to assemble the zeamine antibiotics.

The zeamines are a unique group of antibiotics produced by Serratia plymuthica RVH1 that contain variable hybrid peptide-polyketide moieties connected to a common pentaamino-hydroxyalkyl chain. They exhibit potent activity against a broad spectrum of Gram-positive and Gram-negative bacteria. Here we report a combination of targeted gene deletions, high resolution LC-MS(/MS) analyses, in vitro b...

Thioester reduction and aldehyde transamination are universal steps in actinobacterial polyketide alkaloid biosynthesis† †Electronic supplementary information (ESI) available: Experimental procedures and additional tables, figures, chromatograms and results from biological assays. See DOI: 10.1039/c6sc02803a Click here for additional data file.

Mutual stabilisation between MII 4L6 tetrahedra and MIIX4 2– metallate guests† †Electronic supplementary information (ESI) available: Full experimental procedures, NMR, ESI-MS and UV-Vis spectra and details of the calculated void volumes and CIFS. CCDC 1047195–1047198. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c5sc01083g Click here for additional data file. Click here for additional data file.

Self-assembling α,γ-cyclic peptides that generate cavities with tunable properties† †Electronic supplementary information (ESI) available: Synthetic experimental details, DFT calculation details, additional figures (relevant NMR data) and schemes (synthetic schemes for peptide and amino acid preparation). CCDC 1400134. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c5sc03187g Click here for additional data file. Click here for additional data file.

Watasemycin biosynthesis in Streptomyces venezuelae: thiazoline C-methylation by a type B radical-SAM methylase homologue† †Electronic supplementary information (ESI) available: Experimental procedures and additional tables, figures, chromatograms and results from biological assays. See DOI: 10.1039/c6sc03533g Click here for additional data file.

Department of Chemistry, University of War [email protected] Kitasato Institute for Life Sciences, Kitasato Tokyo, Japan Department of Molecular Microbiology, Joh E-mail: [email protected] † Electronic supplementary information ( and additional tables, gures, chromatogr See DOI: 10.1039/c6sc03533g ‡ These authors contributed equally. § Current address: Department of Bioeng Diego, USA. ...